We have a SORP BD LSR Fortessa flow cytometer. This machine has five lasers: Blue (488mn), Red (640nm), Violet (405nm), Yellow Green (561nm) and

The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays.

The Fortessa is also the instrument used for cell cycle, cell proliferation, cell membrane potential, and… BD LSRIIs and BD LSR-Fortessa have performance, capabilities, and flexibility never seen before in a benchtop analyzer. The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. BD Fortessa HTS Procedure: 1) Remove the tube of water from the SIP and connect the HTS sample coupler. Leave the support arm to the side.

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A1). b. BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode. 2. Disconnect the air line (green) and fluid line (Blue) from the sheath container. 3. Depressurize the sheath container by pulling up on the vent valve.

The BD LSRFortessa has 16 detectors plus forward- and side-scatter detectors and an additional FSC PMT. The Fortessa is used for analysis only. The Fortessa can analyze up to 16 colors at one time. The Fortessa is also the instrument used for cell cycle, cell proliferation, cell membrane potential, and…

Breakthrough with the BD LSRFortessa™ X-20 Cell Analyzer. The BD LSRFortessa X-20 is a premium flow cytometer that brings endless possibilities to multicolor cell analysis. Designed for customization and optimized for reliability, the BD LSRFortessa family of cell analyzers allow you freedom of choice to achieve your research goals–your way. The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers: UV (355 nm) Violet (405 nm) Blue (488 nm) Y-G (561 nm) Red (640 nm) Fortessa X20 Flow Cytometer Quick guide Operation of the 5 laser Fortessa X20 BD Webinar CS&T System BD Compensation Guide… FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day.

FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day.

With up to four different laser configurations and 14 parameters, the cytometer is fully optimized to work with BD Horizon Brilliant ™ dyes, so you can focus on what matters most—your research.

710/50: BV711, QDot705. 660/20: BV650, QDot655. LSR FORTESSA Becton Dickinson.
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BD, BD Logo and all other trademarks are the property of Becton, Dickinson and Company. ©2006 BD 23-8641-00 BD Biosciences Technical Specifications BD™ LSR II The Most Flexible Flow Cytometer Available. Performance Fluorescence Sensitivity Measurements performed using SPHERO™ Rainbow Calibration Particles RCP-30-5A. The BD special order research program allows customers to configure BD flow cytometers and cell sorters to fit precise research and assay needs. Tailored to the special needs of research at the leading edge of biomedical discovery, the program offers a wide range of choices to help researchers create the ultimate customized instrument for their requirements.

Location: 300 George Street, rooms 345 and 345A. Contact: Lesley.Devine@yale.edu. The Fortessas are within rooms that are designated BSL2 space to FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day.
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The BD FacsAria Fusion is an air-cooled, multi-laser, sorter with the ability to acquire up It houses 5 lasers and has a similar configuration as our BD Fortessa.

Other application- specific lasers are available through the special order program. Class 1 Laser Product. BD LSR Fortessa. An advanced and flexible cell analyser with a High Troughput Sampler option. This instrument can be run 24/7 by trained users as per our Policies. BD LSR Fortessa. A Becton Dickinson LSR Fortessa flow cytometer analyzer is being requested by investigators at Purdue University's university-wide and Cancer Center Shared This instrument is for analysis only and has five lasers (UV, violet, blue, green and red) with 16 fluorescence detectors (two from UV laser, four from violet laser, Creating and working with experiment in BD FACSDiva Software.

Apoptosis was measured on an FACS analyzer (LSR Fortessa, BD Biosciences). Phenotypes analysis of cultured cells was performed on a BD FACS Array

Sound Pair this soft, organic pattern with intimate gettogethers and feel-good food. Prizma Prizma’s wide bowl offers sensory enjoyment by giving the wine enough space to develop its full aromas. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company.

Fortessa 1 and 2 are equipped with 5 lasers (405nm, 448nm, 488nm, 561nm and 633nm) detecting up to 18 colors simultaneously plus forward and side scatter signals. BD FACSDiva software features specific to the HTS. z BD FACSFlow Supply System User’s Guide: Describes the optional automated sheath and waste fluid control system designed for use with the BD™ LSR II and BD LSRFortessa. Chapter 1: About this guide 7 Technical assistance For this reason we have chosen a 5-laser BD Fortessa configuration with similar lasers and fluorescence collection optics to allow for easy porting of analysis experiments to the Aria sorter and to greatly expand the number, scope and complexity of analysis-only experiments for our users who would now be able to use the new requested Fortessa instrument. BD LSRFortessa – 16-colour Analyzer. Lasers. 300mW – 488nm (Blue) solid state 50mW - 561nm (Yellow-Green) solid state 40mW – 640nm (Red) solid state 50mW – 405nm (Violet) solid state.